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TitleChloride Dysregulation through Downregulation of KCC2 Mediates Neuropathic Pain in Both Sexes.
Publication TypeJournal Article
Year of Publication2019
AuthorsMapplebeck, Josiane C. S., Louis-Etienne Lorenzo, Kwan Yeop Lee, Cédric Gauthier, Milind M. Muley, Yves De Koninck, Steven A. Prescott, and Michael W. Salter
JournalCell Rep
Volume28
Issue3
Pagination590-596.e4
Date Published2019 Jul 16
ISSN2211-1247
Abstract

The behavioral features of neuropathic pain are not sexually dimorphic despite sex differences in the underlying neuroimmune signaling. This raises questions about whether neural processing is comparably altered. Here, we test whether the K-Cl co-transporter KCC2, which regulates synaptic inhibition, plays an equally important role in development of neuropathic pain in male and female rodents. Past studies on KCC2 tested only males. We find that inhibiting KCC2 in uninjured animals reproduces behavioral and electrophysiological features of neuropathic pain in both sexes and, consistent with equivalent injury-induced downregulation of KCC2, that counteracting chloride dysregulation reverses injury-induced behavioral and electrophysiological changes in both sexes. These findings demonstrate that KCC2 downregulation contributes equally to pain hypersensitivity in males and females. Whereas diverse (and sexually dimorphic) mechanisms regulate KCC2, regulation of intracellular chloride relies almost exclusively on KCC2. Directly targeting KCC2 thus remains a promising strategy for treatment of neuropathic pain in both sexes.

DOI10.1016/j.celrep.2019.06.059
Alternate JournalCell Rep
PubMed ID31315039