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Discoveries

Graphical abstract - Glasgow et al. 2018

Discovery on memory encoding in the brain

 

Testing of various viral vectors in vivo

  • AAV2/8-CAG-Flex-ChR2-TdTomato
  • AAVDj-EF1a-DIO-MU6-TdTomato-DIO-a5KD
  • AAVDj-EF1a-DIO-MU6-TdTomato-DIO-a5neg
  • AAV9-CAG-Flex-frwd-ChR2-tdTomato
  • AAV9-EF1a-DIO-IC++-eYFP
  • AAV9-CAG-Flex-WGA-eGFP
  • AAV9.CAG.GCaMP6s.WPRE.SV40
  • AAVPHP.eB-Syn-HA-KCC2
  • AAV1237small AAV2/1-Syn-HA-KCC2
  • GFAP-LCK-tdTomato-V5-jGCaMP7f
  • pAAV-hDlx-GqDREADD-dTomato-Fishell-4 - AAV2/5
  • AAV2/9 CAG-DIO-superCLOM-NRN1
  • AAV2/9 Flex CAG-GCaMP6f + m-Ruby2
  • AAV2/9 miniHCRT-ChR2-EYFP
  • AAV9-CAG-FlicR
  • AAV9-Syn-FlicR1
  • AAV8-CAG-FlicR
  • AAV2/1-GFAP-Cyto-iGluSnFr-L
  • AAV2/1-GFAP-Cyto-iGluSnFr-V
  • AAV1-GFAP-Cyto-iGluSnFr-L-CNP-CRIUSMQ
  • AAV1-GFAP-Cyto-iGluSnFr-V-CNP-CRIUSMQ
  • AAV2/1 GFAP-Cyto-iGluSnFr eGFP-Y 
  • AAV2/1 GFAP-Cyto-iGluSnFr Suer Y 
  • hSyn-NlucNG6S
  • AAV2/1 hSyn-NlucNG6S
  • AAV2/5 GFAP-Cyto-iGluSnFr eGFP-Y
  • AAV5 GFAP-Cyto-iGluSnFr eGFP-Y
  • AAV2/9 CGA-NirGECO1 (lot 657)
  • AAVPHP-B CAG-Gcamp6s
  • AAV2/9 hSyn Nir GECO (lot 717)
  • AAV2/PHP-B-AAV CAG-Gcamp6s (lot 396)
  • AAV2/9 CGA-jrGECO1 (lot 421)
  • AAV2/9 CGA-Flex0jrGECO1 (lot 536)
  • AAV9.Syn.NES-jRGECO1a.WPRE.SV40
  • AAV2/PHP.eB-hSyn-GCaMP6s-WPRE
  • AAV2/PHP.eB-Syn.NES-jRGECO1a.WPRE.SV40 (lot 1100)
  • AAV2/php.b-hSyn-FRGeco1a (lot 1209)
  • AAV2/php.b-hSyn-FRGeco1b (lot 1210)
  • AAV2/php.b-hSyn-FRGeco1c (lot 1211)
  • AAV2/php.b-hSyn-FRGeco1d (lot 1212)
  • AAV2/PHP.eB-Syn-Flex-Gcamp6s (lot 1381)

Development & testing of the ‘CLP’ molecule series for normalizing inhibition in different disorders (chronic pain, autism, epilepsy, etc)

Mapplebeck JCS, Lorenzo LE, Lee KY, Gauthier C, Muley MM, De Koninck Y, Prescott SA, Salter MW. Chloride Dysregulation through Downregulation of KCC2 Mediates Neuropathic Pain in Both Sexes. Cell Rep. 2019 Jul 16;28(3):590-596.e4.

Ferrini F, Lorenzo LE, Godin AG, Quang ML, De Koninck Y. Enhancing KCC2 function counteracts morphine-induced hyperalgesia. Sci Rep. 2017 Jun 20;7(1):3870.

It can also be noted that a partnership was developed with Hofmann-La Roche (2013-2015) to improve potency and bioavailability of the best CLP molecules candidates for indications such as autism and pain.

The CNP has also initated discussions with SOVAR (https://www.sovar.com/) for the promotion of CLP molecules (November 2019).

Articles using the tools developed by the Molecular Tools Platform

The authors of the following articles have reported back to us that they used tools developed by the Molecular Tools Platform. This list is non-exhaustive.

  1. Barrett, K. T., Roy, A., Rivard, K. B., Wilson, R. J. A. & Scantlebury, M. H. Vagal TRPV1 activation exacerbates thermal hyperpnea and increases susceptibility to experimental febrile seizures in immature rats. Neurobiology of Disease 119, 172–189 (2018).
  2. Beaudry, H., Daou, I., Ase, A. R., Ribeiro-da-Silva, A. & Séguéla, P. Distinct behavioral responses evoked by selective optogenetic stimulation of the major TRPV1+ and MrgD+ subsets of C-fibers. Pain 158, 2329–2339 (2017).
  3. Chabrat, A. et al. Transcriptional repression of Plxnc1 by Lmx1a and Lmx1b directs topographic dopaminergic circuit formation. Nature Communications 8, 933 (2017).
  4. Chapdelaine, P. et al. Development of an AAV9 coding for a 3XFLAG-TALEfrat#8-VP64 able to increase in vivo the human frataxin in YG8R mice. Gene Ther. 23, 606–614 (2016)
  5. Doucet-Beaupré, H. et al. Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons. Proc. Natl. Acad. Sci. U.S.A. 113, E4387–96 (2016).
  6. Josset, N. et al. Distinct Contributions of Mesencephalic Locomotor Region Nuclei to Locomotor Control in the Freely Behaving Mouse. Current Biology 28, 884–901.e3 (2018).
  7. Petitjean, H. et al. Recruitment of Spinoparabrachial Neurons by Dorsal Horn Calretinin Neurons. CellReports 28, 1429–1438.e4 (2019).
  8. Petitjean, H. et al. Dorsal Horn Parvalbumin Neurons Are Gate-Keepers of Touch-Evoked Pain after Nerve Injury. CellReports 13, 1246–1257 (2015).
  9. Qian, Y. et al. A genetically encoded near-infrared fluorescent calcium ion indicator. Nature Publishing Group 16, 1–12 (2019).