|Title||Distinct intracellular Ca(2+) dynamics regulate apical constriction and differentially contribute to neural tube closure.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Suzuki, Makoto, Masanao Sato, Hiroshi Koyama, Yusuke Hara, Kentaro Hayashi, Naoko Yasue, Hiromi Imamura, Toshihiko Fujimori, Takeharu Nagai, Robert E. Campbell, and Naoto Ueno|
|Date Published||2017 Feb 20|
Early in the development of the central nervous system, progenitor cells undergo a shape change, called apical constriction, that triggers the neural plate to form a tubular structure. How apical constriction in the neural plate is controlled, and contributes to tissue morphogenesis, are not fully understood. In this study, we show that intracellular calcium ions (Ca(2+)) are required for Xenopus neural tube formation, and that there are two types of Ca(2+)-concentration changes, a single-cell and a multicellular wave-like fluctuation, in the developing neural plate. Quantitative imaging analyses revealed that transient increases in Ca(2+) concentration induced cortical F-actin remodeling, apical constriction, and accelerations of the closing movement of the neural plate. We also show that extracellular ATP and N-cadherin participate in the Ca(2+)-induced apical constriction. Furthermore, our mathematical model suggests that the effect of Ca(2+) fluctuations on tissue morphogenesis was independent of its frequency, and fluctuations affecting individual cells were more efficient than those at the multicellular level. We propose that distinct Ca(2+) signaling patterns differentially modulate apical constriction for efficient epithelial folding and this mechanism has broad physiological outcomes.