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TitleA bright and high-performance genetically encoded Ca<sup>2+</sup> indicator based on mNeonGreen fluorescent protein
Publication TypeJournal Article
Year of Publication2020
AuthorsZarowny, Landon, Abhi Aggarwal, Virginia M. S. Rutten, Ilya Kolb, The G. E. N. I. E. project, Ronak Patel, Hsin-Yi Huang, Yu-Fen Chang, Tiffany Phan, Richard Kanyo, Misha Ahrens, Ted W. Allison, Kaspar Podgorski, and Robert E. Campbell
JournalbioRxiv
Pagination2020.01.16.909291
Date Published2020/01/01
Abstract

Genetically encodable calcium ion (Ca2+) indicators (GECIs) based on green fluorescent proteins (GFP) are powerful tools for imaging of cell signaling and neural activity in model organisms. Following almost two decades of steady improvements in the Aequorea victoria GFP (avGFP)-based GCaMP series of GECIs, the performance of the most recent generation (i.e., GCaMP7) may have reached its practical limit due to the inherent properties of GFP. In an effort to sustain the steady progression towards ever-improved GECIs, we undertook the development of a new GECI based on the bright monomeric GFP, mNeonGreen (mNG). The resulting indicator, mNG-GECO1, is 60% brighter than GCaMP6s in vitro and provides comparable performance as demonstrated by imaging Ca2+ dynamics in cultured cells, primary neurons, and in vivo in larval zebrafish. These results suggest that mNG-GECO1 is a promising next-generation GECI that could inherit the mantle of GCaMP and allow the steady improvement of GECIs to continue for generations to come.

URLhttp://biorxiv.org/content/early/2020/01/21/2020.01.16.909291.abstract