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TitleA mouse model of small-vessel disease that produces brain-wide-identified microocclusions and regionally selective neuronal injury.
Publication TypeJournal Article
Year of Publication2015
AuthorsSilasi, Gergely, Jennifer She, Jamie D. Boyd, Songchao Xue, and Timothy H. Murphy
JournalJ Cereb Blood Flow Metab
Volume35
Issue5
Pagination734-8
Date Published2015 May
ISSN1559-7016
Abstract

We developed a mouse model of small-vessel disease where occlusions are produced through endovascular injection of fluorescent microspheres that target ~12 μm diameter penetrating arterioles and can be localized in histology. Using Thy1-GFP transgenic mice, we visualized the impact of microocclusions on neuronal structure. Microocclusions in the hippocampus produce cell loss or neuronal atrophy (~7% of lodged microspheres led to microinfarcts), while axons within white matter tracts, as well as the striatum and thalamus became blebbed or disrupted. Although the neocortex contained more occlusions than other structures, labeled layer 5 neurons were relatively resistant to structural damage, with <2% of the lodged microspheres producing obvious neuronal damage.

DOI10.1038/jcbfm.2015.8
Alternate JournalJ. Cereb. Blood Flow Metab.
PubMed ID25690472
PubMed Central IDPMC4420872
Grant ListMOP-111009 / / Canadian Institutes of Health Research / Canada