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TitleRatiometric biosensors based on dimerization-dependent fluorescent protein exchange.
Publication TypeJournal Article
Year of Publication2015
AuthorsDing, Yidan, Jiao Li, Jhon Ralph Enterina, Yi Shen, Issan Zhang, Paul H. Tewson, Gary C. H. Mo, Jin Zhang, Anne Marie Quinn, Thomas E. Hughes, Dusica Maysinger, Spencer C. Alford, Yan Zhang, and Robert E. Campbell
JournalNat Methods
Volume12
Issue3
Pagination195-8, 3 p following 198
Date Published2015 Mar
ISSN1548-7105
Abstract

We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.

DOI10.1038/nmeth.3261
Alternate JournalNat. Methods
PubMed ID25622108
PubMed Central IDPMC4344385
Grant List5R44NS082222 / NS / NINDS NIH HHS / United States
DP1 CA174423 / CA / NCI NIH HHS / United States
DP1 CA174423 / CA / NCI NIH HHS / United States
MOP 119425 / / Canadian Institutes of Health Research / Canada
MOP 123514 / / Canadian Institutes of Health Research / Canada
NHG 99085 / / Canadian Institutes of Health Research / Canada
R44 NS082222 / NS / NINDS NIH HHS / United States