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TitleUnraveling ultrafast photoinduced proton transfer dynamics in a fluorescent protein biosensor for Ca(2+) imaging.
Publication TypeJournal Article
Year of Publication2015
AuthorsTang, Longteng, Weimin Liu, Yanli Wang, Yongxin Zhao, Breland G. Oscar, Robert E. Campbell, and Chong Fang
JournalChemistry
Volume21
Issue17
Pagination6481-90
Date Published2015 Apr 20
ISSN1521-3765
Abstract

Imaging Ca(2+) dynamics in living systems holds great potential to advance neuroscience and cellular biology. G-GECO1.1 is an intensiometric fluorescent protein Ca(2+) biosensor with a Thr-Tyr-Gly chromophore. The protonated chromophore emits green upon photoexcitation via excited-state proton transfer (ESPT). Upon Ca(2+) binding, a significant population of the chromophores becomes deprotonated. It remains elusive how the chromophore structurally evolves prior to and during ESPT, and how it is affected by Ca(2+) . We use femtosecond stimulated Raman spectroscopy to dissect ESPT in both the Ca(2+) -free and bound states. The protein chromophores exhibit a sub-200 fs vibrational frequency shift due to coherent small-scale proton motions. After wavepackets move out of the Franck-Condon region, ESPT gets faster in the Ca(2+) -bound protein, indicative of the formation of a more hydrophilic environment. These results reveal the governing structure-function relationship of Ca(2+) -sensing protein biosensors.

DOI10.1002/chem.201500491
Alternate JournalChemistry
PubMed ID25761197
Grant List / / Canadian Institutes of Health Research / Canada