|Title||Genetically Encoded Glutamate Indicators with Altered Color and Topology.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Wu, Jiahui, Ahmed S. Abdelfattah, Hang Zhou, Araya Ruangkittisakul, Yong Qian, Klaus Ballanyi, and Robert E. Campbell|
|Journal||ACS Chem Biol|
|Date Published||2018 Jul 20|
Glutamate is one of the 20 common amino acids and of utmost importance for chemically mediated synaptic transmission in nervous systems. To expand the color palette of genetically encoded indicators for glutamate, we used protein engineering to develop a red intensity-based glutamate-sensing fluorescent reporter (R-iGluSnFR1). Manipulating the topology of R-iGluSnFR1, and a previously reported green fluorescent indicator, led to the development of noncircularly permutated (ncp) variants. R- and R-iGluSnFR1 display glutamate affinities of 11 μM and 0.9 μM, respectively. We demonstrate that these glutamate indicators are functional when targeted to the surface of HEK-293 cells. Furthermore, we show that G-iGluSnFR enabled reliable visualization of extrasynaptic glutamate in organotypic hippocampal slice cultures, while R-iGluSnFR can reliably resolve action potential-evoked glutamate transients by electrical field stimuli in cultures of dissociated hippocampal neurons.
|Alternate Journal||ACS Chem. Biol.|