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TitleA genetically encoded near-infrared fluorescent calcium ion indicator.
Publication TypeJournal Article
Year of Publication2019
AuthorsQian, Yong, Kiryl D. Piatkevich, Benedict Mc Larney, Ahmed S. Abdelfattah, Sohum Mehta, Mitchell H. Murdock, Sven Gottschalk, Rosana S. Molina, Wei Zhang, Yingche Chen, Jiahui Wu, Mikhail Drobizhev, Thomas E. Hughes, Jin Zhang, Eric R. Schreiter, Shy Shoham, Daniel Razansky, Edward S. Boyden, and Robert E. Campbell
JournalNat Methods
Volume16
Issue2
Pagination171-174
Date Published2019 Feb
ISSN1548-7105
Abstract

We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca imaging in combination with other optogenetic indicators and actuators.

DOI10.1038/s41592-018-0294-6
Alternate JournalNat. Methods
PubMed ID30664778
PubMed Central IDPMC6393164
Grant ListUF1 NS107680 / NS / NINDS NIH HHS / United States
R21 EY026382 / EY / NEI NIH HHS / United States
R01 DA029639 / DA / NIDA NIH HHS / United States
U01 NS094246 / NS / NINDS NIH HHS / United States
R01 GM104948 / GM / NIGMS NIH HHS / United States
U01 NS090565 / NS / NINDS NIH HHS / United States