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TitleThe Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.
Publication TypeJournal Article
Year of Publication2017
AuthorsRodriguez, Erik A., Robert E. Campbell, John Y. Lin, Michael Z. Lin, Atsushi Miyawaki, Amy E. Palmer, Xiaokun Shu, Jin Zhang, and Roger Y. Tsien
JournalTrends Biochem Sci
Volume42
Issue2
Pagination111-129
Date Published2017 Feb
ISSN0968-0004
KeywordsAnimals, Bacterial Proteins, Humans, Luminescent Proteins, Phytochrome, Protein Engineering
Abstract

Over the past 20 years, protein engineering has been extensively used to improve and modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting them for a fantastic range of applications. FPs have been modified by a combination of rational design, structure-based mutagenesis, and countless cycles of directed evolution (gene diversification followed by selection of clones with desired properties) that have collectively pushed the properties to photophysical and biochemical extremes. In this review, we provide both a summary of the progress that has been made during the past two decades, and a broad overview of the current state of FP development and applications in mammalian systems.

DOI10.1016/j.tibs.2016.09.010
Alternate JournalTrends Biochem. Sci.
PubMed ID27814948
PubMed Central IDPMC5272834
Grant ListP41 GM103412 / GM / NIGMS NIH HHS / United States
U01 NS090590 / NS / NINDS NIH HHS / United States
P50 GM107615 / GM / NIGMS NIH HHS / United States
F32 GM089114 / GM / NIGMS NIH HHS / United States
R01 NS027177 / NS / NINDS NIH HHS / United States
R01 GM086197 / GM / NIGMS NIH HHS / United States