Search form

TitleImage-guided transplantation of single cells in the bone marrow of live animals.
Publication TypeJournal Article
Year of Publication2017
AuthorsTurcotte, Raphaël, Clemens Alt, Judith M. Runnels, Kyoko Ito, Juwell W. Wu, Walid Zaher, Luke J. Mortensen, Lev Silberstein, Daniel C. Côté, Andrew L. Kung, Keisuke Ito, and Charles P. Lin
JournalSci Rep
Volume7
Issue1
Pagination3875
Date Published2017 Jun 20
ISSN2045-2322
Abstract

Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control. The platform was used to deliver single hematopoietic stem cells. The engraftment of transplants was tracked over time, illustrating that the technique can be useful for studying both normal and malignant stem cells in vivo.

DOI10.1038/s41598-017-02896-6
Alternate JournalSci Rep
PubMed ID28634334
PubMed Central IDPMC5478633
Grant ListR01 DK098263 / DK / NIDDK NIH HHS / United States
R01 DK100689 / DK / NIDDK NIH HHS / United States