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TitleImproved methods for chronic light-based motor mapping in mice: automated movement tracking with accelerometers, and chronic EEG recording in a bilateral thin-skull preparation.
Publication TypeJournal Article
Year of Publication2013
AuthorsSilasi, Gergely, Jamie D. Boyd, Jeff Ledue, and Timothy H. Murphy
JournalFront Neural Circuits
Volume7
Pagination123
Date Published2013
ISSN1662-5110
KeywordsAccelerometry, Animals, Brain Mapping, Electroencephalography, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Motor Cortex, Movement, Photic Stimulation, Random Allocation, Skull, Time Factors
Abstract

Optogenetic stimulation of the mouse cortex can be used to generate motor maps that are similar to maps derived from electrode-based stimulation. Here we present a refined set of procedures for repeated light-based motor mapping in ChR2-expressing mice implanted with a bilateral thinned-skull chronic window and a chronically implanted electroencephalogram (EEG) electrode. Light stimulation is delivered sequentially to over 400 points across the cortex, and evoked movements are quantified on-line with a three-axis accelerometer attached to each forelimb. Bilateral maps of forelimb movement amplitude and movement direction were generated at weekly intervals after recovery from cranial window implantation. We found that light pulses of ~2 mW produced well-defined maps that were centered approximately 0.7 mm anterior and 1.6 mm lateral from bregma. Map borders were defined by sites where light stimulation evoked EEG deflections, but not movements. Motor maps were similar in size and location between mice, and maps were stable over weeks in terms of the number of responsive sites, and the direction of evoked movements. We suggest that our method may be used to chronically assess evoked motor output in mice, and may be combined with other imaging tools to assess cortical reorganization or sensory-motor integration.

DOI10.3389/fncir.2013.00123
Alternate JournalFront Neural Circuits
PubMed ID23966910
PubMed Central IDPMC3722499