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TitleInverse-response Ca indicators for optogenetic visualization of neuronal inhibition.
Publication TypeJournal Article
Year of Publication2018
AuthorsZhao, Yufeng, Daniel Bushey, Yongxin Zhao, Eric R. Schreiter, Jed D Harrison, Allan M. Wong, and Robert E. Campbell
JournalSci Rep
Volume8
Issue1
Pagination11758
Date Published2018 Aug 06
ISSN2045-2322
Abstract

We have developed a series of yellow genetically encoded Ca indicators for optical imaging (Y-GECOs) with inverted responses to Ca and apparent dissociation constants (K') ranging from 25 to 2400 nM. To demonstrate the utility of this affinity series of Ca indicators, we expressed the four highest affinity variants (K's = 25, 63, 121, and 190 nM) in the Drosophila medulla intrinsic neuron Mi1. Hyperpolarization of Mi1 by optogenetic stimulation of the laminar monopolar neuron L1 produced a decrease in intracellular Ca in layers 8-10, and a corresponding increase in Y-GECO fluorescence. These experiments revealed that lower K' was associated with greater increases in fluorescence, but longer delays to reach the maximum signal change due to slower off-rate kinetics.

DOI10.1038/s41598-018-30080-x
Alternate JournalSci Rep
PubMed ID30082904
PubMed Central IDPMC6079023
Grant ListMOP-123514 / / Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) /
RGPIN 288338-2010 / / Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada) /
U01 NS094246 / NS / NINDS NIH HHS / United States
Not applicable / / Alberta Innovates - Technology Futures /
Not applicable / / Fondation Brain Canada (Fondation Neuro Canada) /
U01 NS094246 and UO1 NS090565 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
U01 NS090565 / NS / NINDS NIH HHS / United States