List of available molecular tools

List of molecular tools available to order from the Neurophotonics Centre Molecular Tools Platform. Services offered for molecular constructions: Design, cloning and production of molecular constructions in order to produce plasmid expression vectors or to generate transgenic animals (Knock-out or Knock-in animals). Viruses that are not currently in stock, but that are listed here, can be produced in approximately 3 weeks time. If the construct you are interested in is not listed, please email marie-eve.paquet@bcm.ulaval.ca to check if it is possible to get it. Please read our DISCLAIMER at the bottom of this page prior to ordering.

Tools from Karl Deisseroth's lab

If you are using these viruses for the first time, please obtain a Material Transfer Agreement (MTA) by contacting Dr. Karl Deisseroth deissero@stanford.edu and Charu Ramakrishnan charur@stanford.edu. Please submit a copy of your MTA along with your virus order. For a complete description of these constructs, please visit Dr. Deisseroth’s website: http://www.stanford.edu/group/dlab/optogenetics/sequence_info.html.

Constructs available Virus in stock?
1. AAV-EF1a-DIO-EYFP AAV2 (lot 217, lot 226)
2. AAV-EF1a-DIO-mCherry  AAV9 (lot 326) AAV2 (lot 346)
3. AAV-EF1a-DIO-hChR2(H134R)-EYFP AAV2 (lot 227) AAV5 (lot 374) AAV2 (lot 449)
4. AAV-EF1a-DIO-hChR2(H134R)-mCherry AAV9 (lot 433)
5. AAV-EF1a-DIO-eNpHR 3.0-EYFP  
6. AAV-EF1a-DIO-ChETA-EYFP AAV9 (lot 390)
7. AAV-CaMKIIa-EYFP AAV9 (lot 233)
8. AAV-CaMKIIa-mCherry  
9. AAV-CaMKIIa-hChR2(H134R)-EYFP AAV9 (lot 405) AAV5 (lot 289)
10. AAV-CaMKIIa-hChR2(H134R)-mCherry  
11. AAV-CaMKIIa-eNpHR 3.0-EYFP  
12. AAV-hSyn-hChR2(H134R)-EYFP AAV2 (lot 219)  AAV5 (lot 437)
13. AAV-hSyn-hChR2(H134R)-mCherry  
14. AAV-hSyn-eNpHR 3.0-EYFP  
15. pAAV-CaMKIIa-C1V1 (E122T/E162T)-TS-EYFP  
16. pAAV-CaMKIIa-C1V1 (E122T/E162T)-TS-mCherry  
17. pAAV-EF1a-DIO-C1V1 (E122T/E162T)-TS-EYFP AAV9 (lot 207)
18. pAAV-EF1a-DIO-C1V1 (E122T/E162T)-TS-mCherry  
19. pAAV-CaMKIIa-hChR2 (C128S/D156A)-EYFP  
20. pAAV-CaMKIIa-hChR2 (C128S/D156A)-mCherry  
21. pAAV-EF1a-DIO-hChR2 (C128S/D156A)-EYFP  
22. pAAV-EF1a-DIO-hChR2 (C128S/D156A)-mCherry  
23. pAAV-CaMKIIa-hChR2 (E123T/T159C)-EYFP  
24. pAAV-CaMKIIa-hChR2 (E123T/T159C)-mCherry  
25. pAAV-CaMKIIa-hChR2 (E123A)-EYFP-WPRE  
26. pAAV-CaMKIIa-hChR2 (E123A)-mCherry-WPRE  
27. pAAV-EF1a-DIO-hChR2 (E123T/T159C)-EYFP  
28. pAAV-EF1a-DIO-hChR2 (123T/T159C)-mCherry  
29. pAAV-EF1a-DIO-ChR2 (E123A)-EYFP-WPRE  
30. pAAV-EF1a-DIO-ChR2 (E123A)-mCherry-WPRE  
31. pAAV-EF1a-DIO-eNpHR 3.0-mCherry-WPRE AAV1 (lot 85)
32. pAAV-CaMKIIa-eNpHR 3.0-mCherry-WPRE AAV7 (lot 109)
33. pAAV-hSyn-eNpHR 3.0-mCherry-WPRE AAV7(lot 110)
34. pAAV-hSyn ChR2 (E123A)-EYFP-WPRE  
35. pAAV-hSyn-EYFP AAV5 (375) AAV2 (lot 216) AAV8 (lot 213)
36. pAAV-Ef1a-mCherry-IRES-WGA-Cre  
37. pAAV-GFAP-hChR2(H134R)-EYFP  
38. pAAV-GFAP-hChR2(H134R)-mCherry  
77. pAAV-CaMKIIa-EYFP-NRN  
   
INTRSECT tools  
   
Lentivirus constructs available  
39. pLenti-CaMKIIa-OptoA1-mCherry  
40. pLenti-CaMKIIa-OptoB2-mCherry  
41. pLenti-Syn-hChR2(H134R)-EYFP-WPRE  
42. pLenti-mThy1-hChR2(H134R) EYFP  
43. pLenti CaMKIIa-hChR2(E123T/H134R) EYFP (ChETA)  
44. pLenti EF1a-hChR2 (H134R)-EYFP-WPRE  

Tools from Ed Boyden's lab

For a complete description of the tools developed in Dr. Ed Boyden’s laboratory, please visit http://syntheticneurobiology.org/protocols. There is no MTA required for these tools but you are required to acknowledge Dr. Boyden in your publication.

Constructs available Virus in stock?
143-pAAV-CAG-ArchT-GFP  
142-pAAV-Flex-ArchT-GFP AAV9 (lot 453) AAV8 (lot 391)
141-pAAV-Flex-ArchT-tdTomato  
326-pAAV-Syn-ChrimsonR-tdTomato  
319-pAAV-CKII-Chronos-GFP  
458-pAAV-CAG-Flex-frwd-ArChT-EGFP-WPRE AAV8 (lot 343)
   
   
Lentivirus constructs available  
108-FCK-Arch-eGFP-WPRE  
140-FCK-ArchT-GFP  

Tools from Tsien, Roth,Cohen, Oertner

For viruses produced based on constructs from Tsien, Roth,Cohen, Oertner, please obtain an MTA by contacting the principal investigator.

Constructs available Virus in stock?
49-lenti-oChIEF-dtTomato  
273-FCK-Optopatch2  
271 FCK-CheRiff-eGFP  
274-FCK-Optopatch1  
265-pAAV Syn ChR E90R-D156N-T159C 2A tDimer (slow ChloC) AAV9 (lot 313)
266-pAAV Syn ChR E90R-T159C 2A tDimer (fast chloC) AAV9 (lot 314)
246-pAAV-hSYN-DIO-hM3D(Gq)-mCherry (DREADD) AAV9 (lot 426, 201)
247-pAAV-hSYN-DIO-hM4D(Gi)-mCherry (DREADD) AAV9 (lot 401) AAV8 (lot 400, 203)
350-pAAV-hSyn-DIO-mCherry AAV8 (lot 212) AAV9 (lot 295)
351-pAAV-hSyn-HA-hM3D(Gq)-iresmCitrine AAV8 (lot 214)
318-pAAV-CaMKIIa-hM4D(gi)-mCherry  
306-pAAV-CaMKIIa-HA-hM4D(gi)-ires-mCitrine  
317-pAAV-CaMKIIa-hM3D(gq)-mCherry  
305-pAAV-CaMKIIa-HA-hM3D(gq)-ires-mCitrine  
588- pAAV-hSyn-HA-hM4D(Gi)-mCherry AAV8 (lot 474)
587- pAAV-hSyn-HA-hM3D(Gq)-mCherry AAV8 (lot 473)
   
   
   
   
   
   

Tools from Janelia labs

Please contact marie-eve.paquet@bcm.ulaval.ca to complete an MTA for these tools

Constructs available Virus in stock ?
329-pAAV-CAG-Gcamp6f AAV9 (lot187)
411-pAAV-CAG-Gcamp6s AAV9 (lot344) AAVD/j(lot383) AAVDJ/8(385)
499-pAAV-emCBA-Flex-GCaMP6s AAVD/J(lot458)
386-pAAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato AAV9 (lot291) 
553-pAAV-EF1a-DIO-GCaMP6f-P2A-nls-tdTomato  
369-pAAV-SYN-GCaMP6f AAV9 (lot392)
   

CRISPR/CAS9 and shRNA plasmids

Constructs available Virus in stock ?
349-pAAV-U6sgRNA(Sap1)-hSyn-GFP-KASH (Zhang lab)  
359-pBSH1_FE_ScaffoldRSV_GFP (Grimm lab)  
360-pBSU6_FE_ScaffoldRSV_GFP (Grimm lab)  
362-pssV9_CMV12_ShalemCas9_H1gRNA: All in one  (Grimm lab)  
361-pssV9_CMV12_ShalemCas9 (Grimm lab)  

352-LentiCas9-Blast (Zhang lab)

 
348-pKLV-U6gRNA(Bbs1)-PKGpuro2ABFP (Kosuke Yusa lab)  
scAAV-CMV-GFP-pU6-shRNA  
   

Other molecular tools platform constructs

Note that we can produce viruses based on plasmids available through Addgene provided that an MTA has been signed or permission has been granted by the depositing lab.

Constructs available Virus in stock?
75-AAV CBA GAP-EGFP Ires tWGA-dsRed  
146- AAV Flex myr GFP  
219-AAV-CBA-WGA-CRE-WPRE AAV1
242-AAV-CBA-tdTomato-WPRE AAV9
87-AAV-Flex-ChR2-tdTomato-WPRE AAV8
299-AAV-CAG-Cre-P2A-mNeptune AAV1(lot172) AAV9 (lot406)
71-AAV-CAG-Flex-GFP  AAV8 (lot167)
296-AAV-CAG-Flex-tDTomato AAV5(lot171) AAV9 (lot415)
125-AM/CBA-CRE-eGFP-WPRE  
193-ITR-CMV CRE-EGFP AAV9 (lot198)
194-ITR-CMV-mCherry  
228-ITR-CMV-mCherry-IRES-WGA-CRE AAV9(lot115)
227-ITR-CMV-mCherry-IRES-WGA-CRE-WPRE AAV9(lot114)
203-ITR-SYN-(his)GCaMP5-P2A-DsRed  
50-ScAAV-CMV-GAP-EGFP  
122-scAAV-CMV-GFP AAV1(lot137),AAV8 (lots121+116),AAV9(lots117+97),AAV1(lot107)
48-scAAV-CKII-EGFP  
51-scAAV-syn-EGFP  
39-sc AAV emCBA EGFP AAV9 (lot150),AAV1 (lot120+106),AAV8 (lot118)
Lentivirus constructs available  
91-phage-promGFAP-IRES-ZsGreen HIV-21
295-pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE (Hans Clevers)  
62-CMV-myrGFP  
35-CMV-Dsred  
34-CMV-mcherry  
129-CMV-zsGreen HIV44
290-CMV-CRE-P2A-mKate  
289-CMV-CRE-P2A-mNeptune  
Adenovirus constructs available  
139-pShuttle-CMV-GAP-mKate AD94
196-pShuttle-CMV-cre-GFP AD100
160-pShuttle-pCKII-GFP AD101
239-pShuttle-CBA-NpHR3.0-eYFP AD103R
AD77-shuttle-CBA-EGFP AD98

  DISCLAIMER:

  1. THIS BIOLOGICAL MATERIAL IS NOT INTENDED FOR USE IN HUMAN.
  2. This material will be used for teaching or not-for-profit research purposes only.
  3.  Any publication made pursuant to the use of the material shall be made in accordance with the custom of scientific research and shall acknowledge the CRIUSMQ’s (molecular tools platform) contribution.
  4. The Biological Material we are sending you is experimental in nature, and is provided without any representations or warranties whatsoever on an “as is” basis. CRIUSMQ (molecular tools platform) MAKES NO REPRESENTATION OR WARRANTY, WHETHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE MATERIAL AND INFORMATION, INCLUDING ANY REPRESENTATION OR WARRANTY AS TO THE DURABILITY, STORAGE, DISPOSAL, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE OR TO THE NON-INFRINGEMENT OF THE MATERIAL OR INFORMATION ON THE PROPRIETARY RIGHTS OF A THIRD-PARTY. ALSO, CRIUSMQ WILL NOT BE LIABLE FOR ANY INDIRECT, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGE OR LOSS ARISING OUT OF OR RELATED TO THE FOREGOING EVEN IF PROVIDER  HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGE OR LOSS. RECIPIENT ACKNOWLEDGES THAT THE MATERIAL AND INFORMATION ARE OF AN EXPERIMENTAL AND EXPLORATION NATURE, THAT NO PARTICULAR RESULTS CAN BE GUARANTEED.
  5. The use of the material will be made in accordance with the ethic or institutional review board of the related institution and all applicable laws and regulations.