List of available molecular tools

List of molecular tools available to order from the Neurophotonics Centre Molecular Tools Platform. Services offered for molecular constructions: Design, cloning and production of molecular constructions in order to produce plasmid expression vectors or to generate transgenic animals (Knock-out or Knock-in animals). Viruses that are not currently in stock, but that are listed here, can be produced in approximately 3 weeks time. If the construct you are interested in is not listed, please email marie-eve.paquet@bcm.ulaval.ca to check if it is possible to get it. Please read our DISCLAIMER at the bottom of this page prior to ordering.

Make sure to acknowledge the Canadian Neurophotonics Platform and the viral vector facility if you are publishing with tools developped or viruses packaged here !!

Tools from Karl Deisseroth’s lab

If you are using these viruses for the first time, please obtain a Material Transfer Agreement (MTA) by contacting Dr. Karl Deisseroth deissero@stanford.edu and Charu Ramakrishnan charur@stanford.edu. Please submit a copy of your MTA along with your virus order. For a complete description of these constructs, please visit Dr. Deisseroth’s website: http://www.stanford.edu/group/dlab/optogenetics/sequence_info.html.

Constructs available	Virus in stock?
1. AAV-EF1a-DIO-EYFP	AAV2 (lot 217)
2. AAV-EF1a-DIO-mCherry	AAV9 (lot 326) AAV2 (lot 346)
3. AAV-EF1a-DIO-hChR2(H134R)-EYFP	AAV2 (lot 227) AAV5 (lot 374) AAV2 (lot 449) AVV1 (lot 520)
4. AAV-EF1a-DIO-hChR2(H134R)-mCherry	AAV9 (lot 607)
5. AAV-EF1a-DIO-eNpHR 3.0-EYFP	AAV9 (lot 580)
6. AAV-EF1a-DIO-ChETA-EYFP	AAV9 (lot 390)
7. AAV-CaMKIIa-EYFP	AAV9 (lot 638) AAV5 (lot 637)
8. AAV-CaMKIIa-mCherry
9. AAV-CaMKIIa-hChR2(H134R)-EYFP	AAV9 (lot 405, lot 492) AAV5 (lot 289)
10. AAV-CaMKIIa-hChR2(H134R)-mCherry	AAV9 (lot 575) AAV2 (lot 556)
11. AAV-CaMKIIa-eNpHR 3.0-EYFP	AVV9 (lot 660, lot 179)
12. AAV-hSyn-hChR2(H134R)-EYFP	AAV2 (lot 219)  AAV5 (lot 437)
13. AAV-hSyn-hChR2(H134R)-mCherry
14. AAV-hSyn-eNpHR 3.0-EYFP
15. pAAV-CaMKIIa-C1V1 (E122T/E162T)-TS-EYFP
16. pAAV-CaMKIIa-C1V1 (E122T/E162T)-TS-mCherry
17. pAAV-EF1a-DIO-C1V1 (E122T/E162T)-TS-EYFP	AAV9 (lot 207)
18. pAAV-EF1a-DIO-C1V1 (E122T/E162T)-TS-mCherry
19. pAAV-CaMKIIa-hChR2 (C128S/D156A)-EYFP
20. pAAV-CaMKIIa-hChR2 (C128S/D156A)-mCherry
21. pAAV-EF1a-DIO-hChR2 (C128S/D156A)-EYFP
22. pAAV-EF1a-DIO-hChR2 (C128S/D156A)-mCherry
23. pAAV-CaMKIIa-hChR2 (E123T/T159C)-EYFP
24. pAAV-CaMKIIa-hChR2 (E123T/T159C)-mCherry
25. pAAV-CaMKIIa-hChR2 (E123A)-EYFP-WPRE
26. pAAV-CaMKIIa-hChR2 (E123A)-mCherry-WPRE	AAV8 (lot 665)
27. pAAV-EF1a-DIO-hChR2 (E123T/T159C)-EYFP
28. pAAV-EF1a-DIO-hChR2 (123T/T159C)-mCherry
29. pAAV-EF1a-DIO-ChR2 (E123A)-EYFP-WPRE
30. pAAV-EF1a-DIO-ChR2 (E123A)-mCherry-WPRE
31. pAAV-EF1a-DIO-eNpHR 3.0-mCherry-WPRE	AAV1 (lot 85)
32. pAAV-CaMKIIa-eNpHR 3.0-mCherry-WPRE	AAV7 (lot 109)
33. pAAV-hSyn-eNpHR 3.0-mCherry-WPRE	AAV7(lot 110)
34. pAAV-hSyn ChR2 (E123A)-EYFP-WPRE	AAV8 (lot 656)
35. pAAV-hSyn-EYFP	AAV5 (375) AAV2 (lot 216) AAV8 (lot 213)
36. pAAV-Ef1a-mCherry-IRES-WGA-Cre
37. pAAV-GFAP-hChR2(H134R)-EYFP
38. pAAV-GFAP-hChR2(H134R)-mCherry
77. pAAV-CaMKIIa-EYFP-NRN
INTRSECT tools
Lentivirus constructs available
39. pLenti-CaMKIIa-OptoA1-mCherry
40. pLenti-CaMKIIa-OptoB2-mCherry
41. pLenti-Syn-hChR2(H134R)-EYFP-WPRE
42. pLenti-mThy1-hChR2(H134R) EYFP
43. pLenti CaMKIIa-hChR2(E123T/H134R) EYFP (ChETA)
44. pLenti EF1a-hChR2 (H134R)-EYFP-WPRE

Tools from Ed Boyden’s lab

For a complete description of the tools developed in Dr. Ed Boyden’s laboratory, please visit http://syntheticneurobiology.org/protocols. There is no MTA required for these tools but you are required to acknowledge Dr. Boyden in your publication.

Constructs available	Virus in stock?
143-pAAV-CAG-ArchT-GFP
142-pAAV-Flex-ArchT-GFP	AAV9 (lot 453) AAV8 (lot 574)
141-pAAV-Flex-ArchT-tdTomato
326-pAAV-Syn-ChrimsonR-tdTomato	AAV9 (lot 609)
319-pAAV-CKII-Chronos-GFP
458-pAAV-CAG-Flex-frwd-ArChT-EGFP-WPRE	AAV8 (lot 343)
Lentivirus constructs available
108-FCK-Arch-eGFP-WPRE
140-FCK-ArchT-GFP

Tools from Tsien, Roth,Cohen, Oertner

For viruses produced based on constructs from Tsien, Roth,Cohen, Oertner, please obtain an MTA by contacting the principal investigator.

Constructs available	Virus in stock?
49-lenti-oChIEF-dtTomato
273-FCK-Optopatch2
271 FCK-CheRiff-eGFP
274-FCK-Optopatch1
265-pAAV Syn ChR E90R-D156N-T159C 2A tDimer (slow ChloC)	AAV9 (lot 313)
266-pAAV Syn ChR E90R-T159C 2A tDimer (fast chloC)	AAV9 (lot 314)
246-pAAV-hSYN-DIO-hM3D(Gq)-mCherry (DREADD)	AAV9 (lot 426) AAV8 (lot 549, lot 608)
247-pAAV-hSYN-DIO-hM4D(Gi)-mCherry (DREADD)	AAV9 (lot 401, lot 487) AAV8 (lot 400, lot 203, lot 548)
350-pAAV-hSyn-DIO-mCherry	AAV8 (lot 212) AAV9 (lot 295)
351-pAAV-hSyn-HA-hM3D(Gq)-iresmCitrine	AAV8 (lot 214)
318-pAAV-CaMKIIa-hM4D(gi)-mCherry
306-pAAV-CaMKIIa-HA-hM4D(gi)-ires-mCitrine
317-pAAV-CaMKIIa-hM3D(gq)-mCherry
305-pAAV-CaMKIIa-HA-hM3D(gq)-ires-mCitrine
588- pAAV-hSyn-HA-hM4D(Gi)-mCherry	AAV8 (lot 474)
587- pAAV-hSyn-HA-hM3D(Gq)-mCherry	AAV8 (lot 473)

Tools from Janelia labs

Please contact marie-eve.paquet@bcm.ulaval.ca to complete an MTA for these tools

Constructs available	Virus in stock ?
329-pAAV-CAG-Gcamp6f	AAV9 (lot 594)
411-pAAV-CAG-Gcamp6s	AAV9 (lot 271) AAVD/j (lot 383) AAVDJ/8 (lot 385)
499-pAAV-emCBA-Flex-GCaMP6s	AAVD/J (lot 458) AAV9 (lot 354) AAVretro (lot 565)
386-pAAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato	AAV9 (lot 291)
553-pAAV-EF1a-DIO-GCaMP6f-P2A-nls-tdTomato
369-pAAV-SYN-GCaMP6f	AAV9 (lot 696)

CRISPR/CAS9 and shRNA plasmids

Constructs available	Virus in stock ?
349-pAAV-U6sgRNA(Sap1)-hSyn-GFP-KASH (Zhang lab)
359-pBSH1_FE_ScaffoldRSV_GFP (Grimm lab)
360-pBSU6_FE_ScaffoldRSV_GFP (Grimm lab)
362-pssV9_CMV12_ShalemCas9_H1gRNA: All in one  (Grimm lab)
361-pssV9_CMV12_ShalemCas9 (Grimm lab)	AAV9 (lot 446) AAVPHP.B (lot 501)
352-LentiCas9-Blast (Zhang lab)
348-pKLV-U6gRNA(Bbs1)-PKGpuro2ABFP (Kosuke Yusa lab)
scAAV-CMV-GFP-pU6-shRNA

Other molecular tools platform constructs

Note that we can produce viruses based on plasmids available through Addgene provided that an MTA has been signed or permission has been granted by the depositing lab.

Constructs available	Virus in stock?
75-AAV CBA GAP-EGFP Ires tWGA-dsRed
146- AAV Flex myr GFP
219-AAV-CBA-WGA-CRE-WPRE	AAV1 (lot 347)
242-AAV-CBA-tdTomato-WPRE	AAV9 (lot 300)
87-AAV-Flex-ChR2-tdTomato-WPRE	AAVretro (lot 557)
299-AAV-CAG-Cre-P2A-mNeptune	AAV1 (lot 172) AAV9 (lot 406) AAVretro (lot 498)
71-AAV-CAG-Flex-GFP	AAV8 (lot 167) AAVretro (lot 496)
296-AAV-CAG-Flex-tDTomato	AAV5 (lot 171) AAV9 (lot 415, lot 323) AAVretro (lot 564)
125-AM/CBA-CRE-eGFP-WPRE	AVV6 (lot 417) AAV9 (lot 478, lot 517) AAVretro (lot 495)
193-ITR-CMV CRE-EGFP	AAV9 (lot 198) AAV2 (lot 205, lot 628)
194-ITR-CMV-mCherry	AAV2 (lot 623) AAV9 (lot 697)
228-ITR-CMV-mCherry-IRES-WGA-CRE	AAV9 (lot 115) AAV9 (lot 163)
227-ITR-CMV-mCherry-IRES-WGA-CRE-WPRE
203-ITR-SYN-(his)GCaMP5-P2A-DsRed	AAV5 (lot 101)
50-ScAAV-CMV-GAP-EGFP
122-scAAV-CMV-GFP	AAV1 (lot 137, lot 107) AAV8 (lots 121) AAV9 (lots 97 lot 231)  AAVDJ (lot 224)
48-scAAV-CKII-EGFP	AAV9 (lot 236)
51-scAAV-syn-EGFP
39-sc AAV emCBA EGFP	AAV1 (lot 106, lot 488) AAV2 (lot 279) AAV5 (lot 280) AAV7 (lot 297) AAV8 (lot 118) AAV9 (lot 150, lot 184, lot 425) AAVDJ (lot 225, lot 475) aaVDJ/8 (lot 369) AAVPHPB (lot 468)
Lentivirus constructs available
91-phage-promGFAP-IRES-ZsGreen	HIV-21
295-pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE (Hans Clevers)
62-CMV-myrGFP
35-CMV-Dsred
34-CMV-mcherry
129-CMV-zsGreen	HIV44
290-CMV-CRE-P2A-mKate
289-CMV-CRE-P2A-mNeptune
Adenovirus constructs available
139-pShuttle-CMV-GAP-mKate	AD94
196-pShuttle-CMV-cre-GFP	AD100
160-pShuttle-pCKII-GFP	AD101
239-pShuttle-CBA-NpHR3.0-eYFP	AD103R
AD77-shuttle-CBA-EGFP	AD98

  DISCLAIMER:

1. THIS BIOLOGICAL MATERIAL IS NOT INTENDED FOR USE IN HUMAN.
2. This material will be used for teaching or not-for-profit research purposes only.
3.  Any publication made pursuant to the use of the material shall be made in accordance with the custom of scientific research and shall acknowledge the CRIUSMQ’s (molecular tools platform) contribution.
4. The Biological Material we are sending you is experimental in nature, and is provided without any representations or warranties whatsoever on an “as is” basis. CRIUSMQ (molecular tools platform) MAKES NO REPRESENTATION OR WARRANTY, WHETHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE MATERIAL AND INFORMATION, INCLUDING ANY REPRESENTATION OR WARRANTY AS TO THE DURABILITY, STORAGE, DISPOSAL, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE OR TO THE NON-INFRINGEMENT OF THE MATERIAL OR INFORMATION ON THE PROPRIETARY RIGHTS OF A THIRD-PARTY. ALSO, CRIUSMQ WILL NOT BE LIABLE FOR ANY INDIRECT, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGE OR LOSS ARISING OUT OF OR RELATED TO THE FOREGOING EVEN IF PROVIDER  HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGE OR LOSS. RECIPIENT ACKNOWLEDGES THAT THE MATERIAL AND INFORMATION ARE OF AN EXPERIMENTAL AND EXPLORATION NATURE, THAT NO PARTICULAR RESULTS CAN BE GUARANTEED.
5. The use of the material will be made in accordance with the ethic or institutional review board of the related institution and all applicable laws and regulations.