Search form

TitleThe McGill-Mouse-Miniscope platform: A standardized approach for high-throughput imaging of neuronal dynamics during behavior.
Publication TypeJournal Article
Year of Publication2021
AuthorsMosser, Coralie-Anne, Zeeshan Haqqee, Andrés Nieto-Posadas, Keith K. Murai, Stefano Stifani, Sylvain Williams, and Mark P. Brandon
JournalGenes Brain Behav
Volume20
Issue1
Paginatione12686
Date Published2021 Jan
ISSN1601-183X
Abstract

Understanding the rules that govern neuronal dynamics throughout the brain to subserve behavior and cognition remains one of the biggest challenges in neuroscience research. Recent technical advances enable the recording of increasingly larger neuronal populations to produce increasingly more sophisticated datasets. Despite bold and important open-science and data-sharing policies, these datasets tend to include unique data acquisition methods, behaviors, and file structures. Discrepancies between experimental protocols present key challenges in comparing data between laboratories and across different brain regions and species. Here, we discuss our recent efforts to create a standardized and high-throughput research platform to address these issues. The McGill-Mouse-Miniscope (M3) platform is an initiative to combine miniscope calcium imaging with standardized touchscreen-based animal behavioral testing. The goal is to curate an open-source and standardized framework for acquiring, analyzing, and accessing high-quality data of the neuronal dynamics that underly cognition throughout the brain in mice, marmosets, and models of disease. We end with a discussion of future developments and a call for users to adopt this standardized approach.

DOI10.1111/gbb.12686
Alternate JournalGenes Brain Behav
PubMed ID32691490