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TitleMetal clad waveguide (MCWG) based imaging using a high numerical aperture microscope objective.
Publication TypeJournal Article
Year of Publication2017
AuthorsSöllradl, Thomas, Frederic A. Banville, Vincent Chabot, Michael Canva, Michel Grandbois, and Paul G. Charette
JournalOpt Express
Volume25
Issue3
Pagination1666-1679
Date Published2017 Feb 06
ISSN1094-4087
Abstract

Evanescent-field based methods such as surface plasmon resonance (SPR) have been used very effectively for label-free imaging of microscopic biological material in close proximity to a sensing surface. However, the shallow probing depth of SPR (typically less than ~200 nm) can be problematic when imaging relatively thick biological objects such as cells or bacteria. In this paper, we demonstrate how metal-clad waveguides (MCWG) can be used to achieve deeper probing depth compared to SPR while maintaining good imaging spatial resolution. Comparative numerical simulations of imaging spatial resolution versus probing depth are shown for a number of common SPR, long-range SPR, and MCWG configurations, demonstrating that MCWG offer the best compromise between resolution and depth for imaging thick biological objects. Experimental results of synthetic target and live cell imaging are shown that validate the numerical simulations and demonstrate the capabilities of the method.

DOI10.1364/OE.25.001666
Alternate JournalOpt Express
PubMed ID29519021