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TitleMolecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes.
Publication TypeJournal Article
Year of Publication2017
AuthorsRomanov, Roman A., Amit Zeisel, Joanne Bakker, Fatima Girach, Arash Hellysaz, Raju Tomer, Alán Alpár, Jan Mulder, Frédéric Clotman, Erik Keimpema, Brian Hsueh, Ailey K. Crow, Henrik Martens, Christian Schwindling, Daniela Calvigioni, Jaideep S. Bains, Zoltán Máté, Gábor Szabó, Yuchio Yanagawa, Ming-Dong Zhang, Andre Rendeiro, Matthias Farlik, Mathias Uhlén, Peer Wulff, Christoph Bock, Christian Broberger, Karl Deisseroth, Tomas Hökfelt, Sten Linnarsson, Tamas L. Horvath, and Tibor Harkany
JournalNat Neurosci
Volume20
Issue2
Pagination176-188
Date Published2017 Feb
ISSN1546-1726
KeywordsAnimals, Dopamine, Dopaminergic Neurons, Hypothalamus, Immunohistochemistry, Mice, Inbred C57BL, Mice, Transgenic, Neuropeptides, Neurotransmitter Agents, Suprachiasmatic Nucleus, Synaptic Transmission, Tyrosine 3-Monooxygenase
Abstract

The hypothalamus contains the highest diversity of neurons in the brain. Many of these neurons can co-release neurotransmitters and neuropeptides in a use-dependent manner. Investigators have hitherto relied on candidate protein-based tools to correlate behavioral, endocrine and gender traits with hypothalamic neuron identity. Here we map neuronal identities in the hypothalamus by single-cell RNA sequencing. We distinguished 62 neuronal subtypes producing glutamatergic, dopaminergic or GABAergic markers for synaptic neurotransmission and harboring the ability to engage in task-dependent neurotransmitter switching. We identified dopamine neurons that uniquely coexpress the Onecut3 and Nmur2 genes, and placed these in the periventricular nucleus with many synaptic afferents arising from neuromedin S(+) neurons of the suprachiasmatic nucleus. These neuroendocrine dopamine cells may contribute to the dopaminergic inhibition of prolactin secretion diurnally, as their neuromedin S(+) inputs originate from neurons expressing Per2 and Per3 and their tyrosine hydroxylase phosphorylation is regulated in a circadian fashion. Overall, our catalog of neuronal subclasses provides new understanding of hypothalamic organization and function.

DOI10.1038/nn.4462
Alternate JournalNat. Neurosci.
PubMed ID27991900