Title | Multimodal virtual histology of rabbit vocal folds by nonlinear microscopy and nano computed tomography. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Kazarine, Alexei, Ksenia Kolosova, Angelica A. Gopal, Huijie Wang, Rui Tahara, Almoaidbellah Rammal, Karen Kost, Luc Mongeau, Nicole Y. K. Li-Jessen, and Paul W. Wiseman |
Journal | Biomed Opt Express |
Volume | 10 |
Issue | 3 |
Pagination | 1151-1164 |
Date Published | 2019 Mar 01 |
ISSN | 2156-7085 |
Abstract | Human vocal folds (VFs) possess a unique anatomical structure and mechanical properties for human communication. However, VFs are prone to scarring as a consequence of overuse, injury, disease or surgery. Accumulation of scar tissue on VFs inhibits proper phonation and leads to partial or complete loss of voice, with significant consequences for the patient's quality of life. VF regeneration after scarring provides a significant challenge for tissue engineering therapies given the complexity of tissue microarchitecture. To establish an effective animal model for VF injury and scarring, new histological methods are required to visualize the wound repair process of the tissue in its three-dimensional native environment. In this work, we propose the use of a combination of nonlinear microscopy and nanotomography as contrast methods for virtual histology of rabbit VFs. We apply these methods to rabbit VF tissue to demonstrate their use as alternatives to conventional VF histology that may enable future clinical studies of this injury model. |
DOI | 10.1364/BOE.10.001151 |
Alternate Journal | Biomed Opt Express |
PubMed ID | 30891336 |
PubMed Central ID | PMC6420294 |