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TitleOptogenetic control with a photocleavable protein, PhoCl.
Publication TypeJournal Article
Year of Publication2017
AuthorsZhang, Wei, Alexander W. Lohman, Yevgeniya Zhuravlova, Xiaocen Lu, Matthew D. Wiens, Hiofan Hoi, Sine Yaganoglu, Manuel A. Mohr, Elena N. Kitova, John S. Klassen, Periklis Pantazis, Roger J. Thompson, and Robert E. Campbell
JournalNat Methods
Date Published2017 Apr
KeywordsConnexins, Directed Molecular Evolution, Green Fluorescent Proteins, HEK293 Cells, HSP90 Heat-Shock Proteins, Humans, Luminescent Proteins, Nerve Tissue Proteins, Nuclear Localization Signals, Optogenetics, Patch-Clamp Techniques, Photochemistry, Protein Engineering, Recombinant Proteins, Viral Nonstructural Proteins

To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.

Alternate JournalNat. Methods
PubMed ID28288123