|Title||Optogenetic control with a photocleavable protein, PhoCl.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Zhang, Wei, Alexander W. Lohman, Yevgeniya Zhuravlova, Xiaocen Lu, Matthew D. Wiens, Hiofan Hoi, Sine Yaganoglu, Manuel A. Mohr, Elena N. Kitova, John S. Klassen, Periklis Pantazis, Roger J. Thompson, and Robert E. Campbell|
|Date Published||2017 Apr|
|Keywords||Connexins, Directed Molecular Evolution, Green Fluorescent Proteins, HEK293 Cells, HSP90 Heat-Shock Proteins, Humans, Luminescent Proteins, Nerve Tissue Proteins, Nuclear Localization Signals, Optogenetics, Patch-Clamp Techniques, Photochemistry, Protein Engineering, Recombinant Proteins, Viral Nonstructural Proteins|
To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.
|Alternate Journal||Nat. Methods|