The Leica VT1200S automatic vibrating blade microtome (vibratome) is designed to section fixed or unfixed tissue at a thickness ranging between 30 and 400µm.
The vertical deflection can be adusted using the “vibrocheck”, providing best results.
A “minichiller” controls the temperature of the buffer during sectioning.
The Q150T E is a high vacuum carbon coater, ideal to obtain highly stable carbon films allowing a better adhesion of the specimen to the grid, before examination under the electron microscope (TEM).
High vacuum carbon coating ideal for transmission electron microscopy.
Precise thickness control using the film thickness monitor option.
“Intelligent” recognition system, automatically detects the type of coating.
Simple, fast and reproductible.
Suitable for carbon coating and other metal evaporation.
The Vitrobot is an automated vitrification device for plunge-freezing of aqueous suspensions. A controlled environment prevents artefacts. Grids transfer from the vitrification medium into liquid nitrogen is automatic, ensuring reproducible sample freezing and high sample throughput.
Temperature, number of blottings, blotting pressure, and drain time can be programmed.
Easy and flexible instrument control.
Semi-automated grid transfer.
Frozen samples can be stored at the NEUROPHOTONICS CENTRE in the cryo-biological storage system.
Company: Leica Microsystems Model: EM PACT2 and EM RTS
The high-pressure freezer is used to prepare biological samples by cryo-fixation (samples up to 200µm in thickness).
Live cell carrier is available for cell cultures. Cells can be first examined with a light microscope and then rapidly frozen (in only five seconds) with the EM PACT2-EM RTS.
A microbiopsy system is available for rapid transfer of tissue into the EMPACT2.
The EM PACT2 can be easily transported.
Frozen samples can be stored at the Neurophotonics Centre in the cryo-biological storage system.
Company: Leica Microsystems Ultramicrotome models: Leica Ultracut EM UCT Leica Ultracut EM UC7 Cryo-Chamber: Leica EM FC
Ultramicrotomes are used to prepare semi and ultra-thin sections of biological samples (thickness ranging between 50-200 nm) that can be examined under the electron microscope
Leica Ultracut UCT
A cryo-chamber is available to cut biological samples at low temperature (-15°C to – 185°C). Frozen ultra and semi-thin sections can be stored at the Neurophotonics Centre in a cryo-biological storage system.
Company: Philips Model: Tecnai 12, 120 kV Magnification: 19.5 X à 300 000 X Acquisition software: Tecnai UI version 2.1.8, FEI company Analysis software: AnalySIS version 3.2, Soft Imaging System GmbH Digital Camera: Mega View I
Each user must have gone through a complete training in order to use any equipment of the facility.
Reservation must be addressed to:
Martin Parent, Ph.D Institut universitaire en santé mentale de Québec 2601, chemin de la Canardière, F-6500 Tel.:(418) 663-5747 E-mail:martin.parent@crulrg.ulaval.ca
Reservation
Users who have received a complete training are allowed to reserve. NEUROPHOTONICS CENTRE members have priority over external users.
To reserve, please contact:
Martin Parent, Ph.D Institut universitaire en santé mentale de Québec 2601, chemin de la Canardière, F-6500 Tel.:(418) 663-5747 E-mail:martin.parent@crulrg.ulaval.ca
Fee schedule
Instrument
Neurophotonics fee
Outside fee
FEI TECNAI 12, 120 kV, TEM
45$ / h without assistance
67.80 / h without assistance
60$ / h with assistance
90$ / h with assistance
Leica Ultramicrotome1
10$ / h
15$ / h
Leica Cryo-Ultramicrotome1
20$ / h
30$ / h
Leica Microbiopsy Transfer System
15$ / h
30$ / h
Leica EMPACT2-EM RTS High Pressure Freezer
30$ / h
50$ / h
Leica EM AFS Automatic Freeze Substitution
80$ / run
120$ / run
Evaporator Coater, Quorum Q150T E
20$ / run
35$ / run
Leica VT1200S, Vibrating Blade Microtome
20$ / h
35$ / h
FEI Vitrobot Vitrification Robot (Plunge Freezer)
30$ / h
45$ / h
Services
Neurophotonics fee
Outside fee
Embedding
30$ / sample
45$ / sample
Sectioning
30$ / sample
45$ / sample
Cryo-sectioning
100$ / sample
150$ / sample
Cryo-sample preparation (plunge freezing, including material, grids and storage box)
80$ / h
120$ / h
Training and Assistance
Neurophotonics fee
Outside fee
Technical assistance
50$ / h
75$ / h
Transmission electron microscope training
50$ / h, 3 h minimum
75$ / h, 3 h minimum
Ultramicrotome training1
50$ / h, 3 h minimum
75$ / h, 3 h minimum
Supplies
Neurophotonics fee
Outside fee
Grids (Cu/Ni) with formvar film
0,50$ / grid
1$ / grid
Grids (Cu/Ni) with formvar film and carbon
1$ / grid
2$ / grid
Grids (Cu/Ni)
30$ / vial
35$ / vial
Grid Box
20$ / each
30$ / each
Cryo-storage box (contains 4 grids)
10$ / each
15$ / each
Package deal
Neurophotonics fee
Outside fee
Transmission Electron Microscope, FEI TECNAI 12, 120 kV2
1 500$ / 78 h 3 000$ / 156 h
3 000$ / 78 h 6 000$ / 156 h
Leica Ultramicrotome1
2 500$ / year
5 000$ / year
Leica Cryo-ultramicrotome1
3 500$ / year
7 000$ / year
Leica EMPACT2-EM RTS High Pressure Freezer
2 500$ / year
5 000$ / year
Leica VT1200S, Vibrating Blade Microtome
1 500$ / year
3 000$ / year
1Users provide their own diamond knife 2 3 hours per week. Additional hours will be charged at regular rates. Training and supplies are not included.
The electron microscopy facility located at the Neurophotonics Centre provides advanced technical tools for transmission electron microscopy.
Our staff offers complete processing of biological samples, including cryo-fixation, embedding and semi and ultra-thin sectioning with ultra-microtome. Complete training for each equipment is also available.
Martin Parent, Ph.D Institut universitaire en santé mentale de Québec 2601, chemin de la Canardière, F-6500 Québec (Québec), G1J 2G3 Tel.:(418) 663-5747 E-mail:martin.parent@cervo.ulaval.ca
