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Neurophotonics.Ca

at the interface of physics & neuroscience

Cell Sorting Platform – Fees

 CERVO
Investigator
AcademicCompanies
Flow Cytometry90$140$220$
Cell Sorting110$180$250$
Protocols and analysis60$75$
hourly rate

* hourly rate

New Fees

• Charge will be by 30 minute period
• Cancellation fees corresponding to 1h30 will be charged if cancelation is made within 24 hours
• Late fees will start to be charged for 30 minute delay or more.
• An additionnal fee of 60$ will be added for a sterile sort for sorter preparation
• If sample preparation is needed, a fee of 50$ will be charges
• Possibility of buying lab materials (ex.: FACS tube, compensation beads, cell strainer tubes…) Charge will apply.
• 1 hour fees will apply when sort or analysis starts after 17h00 or during weekend

Last updated April 2023

Cell sorting | Services and Applications

SERVICES

  • Consulting and protocol development for both flow cytometry and cell sorting applications
  • Advisement on the choice of the fluorochromes
  • Instrument operation
  • Various cell population analysis
    • Neurons
    • Astrocytes
    • Immune cells
  • Data analysis and preparation of figures for scientific articles or grants

APPLICATIONS

  • Phenotyping
  • Sorting
  • Cell cycle evaluation
  • Viability and apoptosis analysis
  • Screening
  • Titration based on endogenous fluorescence or immunostaining

Various samples may be analysed :

  • freshly dissociated cells
  • cultured cells (treated or not, transfected, transduced, etc.)
  • immunostained cells
image_3

Cell Sorting Equipment

Specific informations on the FACS Aria II (BD Biosciences) The Cell Sorting Platform is equipped with a FACS AriaII (BD Biosciences) showing the following features :

  • 3 lasers :
    • Sapphire Blue 488nm
    • HeNe Red 633 nm
    • Yellow-green 561nm
  • PMTs and photodiodes allowing the detection of 12 parameters, including 10 different fluorochromes
  • Cooling unit
  • Various nozzle sizes : 70µ, 85µ et 100µ
  • Sample pressure control
  • Simultaneous sorting
    • in tubes (1.5, 5 ou 15mL)
    • in plates (6, 12, 24 ou 96 wells)
    • on slides
  • Software for data analysis : FACS Diva 6.1.3 et FlowJo 10.8.2
image_2

Cell Sorting Platform

Example of the spinothalamic core of neurons expressing the GFP protein after transduction with an adenoviral vector which may be sorted by FACS (photos: Karine Bachand)
Example of the spinothalamic core of neurons expressing the GFP protein after transduction with an adenoviral vector which may be sorted by FACS (photos: Karine Bachand)

Cell Sorting Platform Team

  • Scientific Advisor : Marie-Ève Paquet, PhD
  • Coordination and instrument operation: Dominic Bastien, PhD

Physical and optical properties used to sort neurons

The IUSMQ Cell Sorting Platform is highly specialized in analysis and sorting of neurons and cells isolated from the nervous system. Various cell sub-populations may be individually studied upon their physical properties (size and granularity) as well as their optical properties (fluorescent staining or expression). We use a cell sorter from BD Biosciences (FACS Aria II) capable of detecting 12 simultaneous parameters including 10 different fluorochromes. In addition of the instrument operation, we offer a consulting service for the :

  • protocol development related to the cell dissociation step
  • sample immunostaining
  • choice of fluorochromes
  • cell sorting
  • data analysis (2 available softwares : DiVA and FlowJo)
  • preparation of figures for scientific articles or grants

Contact

If you have any questions, including sample preparation, or if you want to make a reservation for the instrument, please don’t hesitate to contact us by e-mail at plateformetricervo@neurophotonics.ca or by phone at the 418 663-5741 ext 26895