Search form

TitleQuantification of stored red blood cell fluctuations by time-lapse holographic cell imaging.
Publication TypeJournal Article
Year of Publication2018
AuthorsJaferzadeh, Keyvan, Inkyu Moon, Manon Bardyn, Michel Prudent, Jean-Daniel Tissot, Benjamin Rappaz, Bahram Javidi, Gerardo Turcatti, and Pierre Marquet
JournalBiomed Opt Express
Date Published2018 Oct 01

We propose methods to quantitatively calculate the fluctuation rate of red blood cells with nanometric axial and millisecond temporal sensitivity at the single-cell level by using time-lapse holographic cell imaging. For this quantitative analysis, cell membrane fluctuations (CMFs) were measured for RBCs stored at different storage times. Measurements were taken over the whole membrane for both the ring and dimple sections separately. The measurements show that healthy RBCs that maintain their discocyte shape become stiffer with storage time. The correlation analysis demonstrates a significant negative correlation between CMFs and the sphericity coefficient, which characterizes the morphological type of erythrocyte. In addition, we show the correlation results between CMFs and other morphological properties such as projected surface area, surface area, mean corpuscular volume, and mean corpuscular hemoglobin.

Alternate JournalBiomed Opt Express
PubMed ID30319898
PubMed Central IDPMC6179419