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TitleRatiometric Detection of Nerve Agents by Coupling Complementary Properties of Silicon-Based Quantum Dots and Green Fluorescent Protein.
Publication TypeJournal Article
Year of Publication2019
AuthorsRobidillo, Christopher Jay T., Sophia Wandelt, Rochelin Dalangin, Lijuan Zhang, Haoyang Yu, Alkiviathes Meldrum, Robert E. Campbell, and Jonathan G. C. Veinot
JournalACS Appl Mater Interfaces
Volume11
Issue36
Pagination33478-33488
Date Published2019 Sep 11
ISSN1944-8252
Abstract

Ratiometric photoluminescent detection of the toxicologically potent organophosphate ester nerve agents paraoxon (PX) and parathion (PT) using the complementary optical and chemical properties of the long Stokes shift green fluorescent protein variant, mAmetrine1.2 (mAm), and red-emitting silicon-based quantum dots (SiQDs) is reported. PX and PT selectively quench SiQD photoluminescence (PL) through a dynamic quenching mechanism, thereby, facilitating the development of a ratiometric sensor platform that shows micromolar limits of detection for PX and PT and that is unaffected by the presence of common inorganic and organic interferents. As a part of the present study, we also demonstrate that the paper-based sensors derived from mAm and SiQDs detect PX and PT at concentrations as low as 5 μM using a readily available commercial color analysis smartphone "app". The ratiometric sensor reported herein can potentially be used for the convenient and rapid on-site detection and quantification of PX and PT in real-world samples.

DOI10.1021/acsami.9b10996
Alternate JournalACS Appl Mater Interfaces
PubMed ID31414591