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TitleThe Role of Amino Acids in Neurotransmission and Fluorescent Tools for Their Detection.
Publication TypeJournal Article
Year of Publication2020
AuthorsDalangin, Rochelin, Anna Kim, and Robert E. Campbell
JournalInt J Mol Sci
Volume21
Issue17
Date Published2020 Aug 27
ISSN1422-0067
Abstract

Neurotransmission between neurons, which can occur over the span of a few milliseconds, relies on the controlled release of small molecule neurotransmitters, many of which are amino acids. Fluorescence imaging provides the necessary speed to follow these events and has emerged as a powerful technique for investigating neurotransmission. In this review, we highlight some of the roles of the 20 canonical amino acids, GABA and β-alanine in neurotransmission. We also discuss available fluorescence-based probes for amino acids that have been shown to be compatible for live cell imaging, namely those based on synthetic dyes, nanostructures (quantum dots and nanotubes), and genetically encoded components. We aim to provide tool developers with information that may guide future engineering efforts and tool users with information regarding existing indicators to facilitate studies of amino acid dynamics.

DOI10.3390/ijms21176197
Alternate JournalInt J Mol Sci
PubMed ID32867295
PubMed Central IDPMC7503967
Grant ListFS-154310 / CAPMC / CIHR / Canada
RGPIN-2018-04364 / / Natural Sciences and Engineering Research Council of Canada /
Grants-in-Aid for Scientific Research / / Japan Society for the Promotion of Science /