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TitleTargeted ischemic stroke induction and mesoscopic imaging assessment of blood flow and ischemic depolarization in awake mice.
Publication TypeJournal Article
Year of Publication2017
AuthorsBalbi, Matilde, Matthieu P. Vanni, Gergely Silasi, Yuki Sekino, Luis Bolanos, Jeffrey M. LeDue, and Timothy H. Murphy
JournalNeurophotonics
Volume4
Issue3
Pagination035001
Date Published2017 Jul
ISSN2329-423X
Abstract

Despite advances in experimental stroke models, confounding factors such as anesthetics used during stroke induction remain. Furthermore, imaging of blood flow during stroke is not routinely done. We take advantage ofbihemispheric transcranial windows for longitudinal mesoscopic imaging of cortical function to establish a protocol for focal ischemic stroke induction in target brain regions using photothrombosis in awake head-fixed mice. Our protocol does not require any surgical steps at the time of stroke induction or anesthetics during either head fixation or photoactivation. In addition, we performed laser speckle contrast imaging and wide-field calcium imaging to reveal the effect of cortical spreading ischemic depolarization after stroke in both anesthetized and awake animals over a spatial scale encompassing both hemispheres. With our combined approach, we observed ischemic depolarizing waves (3 to [Formula: see text]) propagating across the cortex 1 to 5 min after stroke induction in genetically encoded calcium indicator mice. Measures of blood flow by laser speckle were correlated with neurological impairment and lesion volume, suggesting a metric for reducing experimental variability. The ability to follow brain dynamics immediately after stroke as well as during recovery may provide a valuable guide to develop activity-dependent therapeutic interventions to be performed shortly after stroke induction.

DOI10.1117/1.NPh.4.3.035001
Alternate JournalNeurophotonics
PubMed ID28721356
PubMed Central IDPMC5512458