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TitleTNF receptor-associated factor 6 interacts with ALS-linked misfolded superoxide dismutase 1 and promotes aggregation.
Publication TypeJournal Article
Year of Publication2020
AuthorsSemmler, Sabrina, Myriam Gagné, Pranav Garg, Sarah R. Pickles, Charlotte Baudouin, Emeline Hamon-Keromen, Laurie Destroismaisons, Yousra Khalfallah, Mathilde Chaineau, Elise Caron, Andrew N. Bayne, Jean-François Trempe, Neil R. Cashman, Alexandra T. Star, Arsalan S. Haqqani, Thomas M. Durcan, Elizabeth M. Meiering, Janice Robertson, Nathalie Grandvaux, Steven S. Plotkin, Heidi M. McBride, and Christine Vande Velde
JournalJ Biol Chem
Date Published2020 03 20
KeywordsAmyotrophic Lateral Sclerosis, Animals, Antibodies, Cell Line, Disease Models, Animal, Mitochondria, Mutagenesis, Site-Directed, NF-kappa B, Protein Aggregates, Protein Folding, Rats, Rats, Transgenic, RNA Interference, RNA, Small Interfering, Superoxide Dismutase-1, TNF Receptor-Associated Factor 6, Ubiquitination

Amyotrophic lateral sclerosis (ALS) is a fatal disease, characterized by the selective loss of motor neurons leading to paralysis. Mutations in the gene encoding superoxide dismutase 1 (SOD1) are the second most common cause of familial ALS, and considerable evidence suggests that these mutations result in an increase in toxicity due to protein misfolding. We previously demonstrated in the SOD1 rat model that misfolded SOD1 exists as distinct conformers and forms deposits on mitochondrial subpopulations. Here, using SOD1 rats and conformation-restricted antibodies specific for misfolded SOD1 (B8H10 and AMF7-63), we identified the interactomes of the mitochondrial pools of misfolded SOD1. This strategy identified binding proteins that uniquely interacted with either AMF7-63 or B8H10-reactive SOD1 conformers as well as a high proportion of interactors common to both conformers. Of this latter set, we identified the E3 ubiquitin ligase TNF receptor-associated factor 6 (TRAF6) as a SOD1 interactor, and we determined that exposure of the SOD1 functional loops facilitates this interaction. Of note, this conformational change was not universally fulfilled by all SOD1 variants and differentiated TRAF6 interacting from TRAF6 noninteracting SOD1 variants. Functionally, TRAF6 stimulated polyubiquitination and aggregation of the interacting SOD1 variants. TRAF6 E3 ubiquitin ligase activity was required for the former but was dispensable for the latter, indicating that TRAF6-mediated polyubiquitination and aggregation of the SOD1 variants are independent events. We propose that the interaction between misfolded SOD1 and TRAF6 may be relevant to the etiology of ALS.

Alternate JournalJ Biol Chem
PubMed ID32029478
PubMed Central IDPMC7086032