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Neurophotonics.Ca

at the interface of physics & neuroscience

Cell Sorting Platform

Example of the spinothalamic core of neurons expressing the GFP protein after transduction with an adenoviral vector which may be sorted by FACS (photos: Karine Bachand)
Example of the spinothalamic core of neurons expressing the GFP protein after transduction with an adenoviral vector which may be sorted by FACS (photos: Karine Bachand)

Cell Sorting Platform Team

  • Scientific Advisor : Marie-Ève Paquet, PhD
  • Coordination and instrument operation: Dominic Bastien, PhD

Physical and optical properties used to sort neurons

The IUSMQ Cell Sorting Platform is highly specialized in analysis and sorting of neurons and cells isolated from the nervous system. Various cell sub-populations may be individually studied upon their physical properties (size and granularity) as well as their optical properties (fluorescent staining or expression). We use a cell sorter from BD Biosciences (FACS Aria II) capable of detecting 12 simultaneous parameters including 10 different fluorochromes. In addition of the instrument operation, we offer a consulting service for the :

  • protocol development related to the cell dissociation step
  • sample immunostaining
  • choice of fluorochromes
  • cell sorting
  • data analysis (2 available softwares : DiVA and FlowJo)
  • preparation of figures for scientific articles or grants

Contact

If you have any questions, including sample preparation, or if you want to make a reservation for the instrument, please don’t hesitate to contact us by e-mail at plateformetricervo@neurophotonics.ca or by phone at the 418 663-5741 ext 26895

Cell sorting

Electron Microscopy